高手帮忙把摘要翻译成英文，谢谢了！
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发布时间：2022-04-20 08:09
共2个回答
热心网友
时间：2022-07-14 04:26
Objective: to use electricity spinning method for preparation of e. coli phage, molecularly imprinted polymer membranes of the template. In this test requirements with the whole process of scientific research, including data collection, the subject designing, experimental operation and professional writing the thesis. While improving students' scientific thinking ability and practical ability.
Methods: using mpeg precipitation + chloroform extraction smoke concentration purification phage reach 1012 / ml, concentration of PVA by adjusting voltage, high flow rate, and spinning, the preparation conditions after film by scanning electron microscope (SEM) spinning solution concentration, flow, voltage, high on spinning fiber diameter and the morphology of the influence. Using the electrostatic spinning technology in preparation for the conditions for the "shell" with PVA, escherichia coli phage as "core" of molecularly imprinted membrane, and through the double plaque method of observing AGAR bite after encapsulation testing of bioactive phage.
Results: the successful condensed phage reach 1012 / ml. In the process of preparation conditions with water groping for solvent for 7% PVA concentration (w/w) of membrane 2, Before joining ethanol and SM, spinning is unstable, lots of broken wire and beads, liquid membrane, not because of time, not continue, experiment which terminates. Turn on the morphology, PVA spinning fiber diameter with PVA concentration, the relationship between voltage
Conclusions: (1) by the preparation of several membranes observed results show that, with the increase of spinning solution concentration, fiber diameter increase graally, the distribution of fiber diameter with, With the increase of fiber diameter, voltage did not change significantly.
2, the experiment of polymer matrix phage and spinning compatibility of PVA were evaluated.
[key] : a phage maximizing electrostatic spinning PVA
热心网友
时间：2022-07-14 04:26
[Abstract]: Objective: To make use of electric spinning method for preparation of E. coli phage template molecular imprinting polymer film. Requirements in this experiment are familiar with the whole process of scientific research, including data collection, the subject design, the experimental operation and the writing of professional papers. At the same time improve the thinking ability of students to scientific research and practical hands-capacity.
Methods: PEG precipitation + chloroform extraction purification of phage enrichment after reaching 1012/ml, by adjusting the concentration of PVA, flow, voltage, and other conditions of the high spinning, film prepared by scanning electron microscopy (SEM) observation of spin silk solution concentration, flow rate, voltage, and other high fiber spinning conditions on morphology and diameter of the impact. Electrospinning technology use in the above conditions and explore the preparation of a PVA for the "shell", E. coli phage as a "core" of the molecularly imprinted membrane, and through observation of double-layer agar method Plaque packaged phage detection of biological activity.
Results: The success of purification of phage concentration to achieve the above 1012/ml. Groping in the process of the preparation conditions with water as the solvent for the PVA concentration of 7% (w / w) of the membrane 2; in the SM by adding ethanol and, after spinning a very unstable and a large number of broken wires, beads, drip phenomenon, not film, e to time reasons, did not continue, the resulting termination of the experiment. Turn to the morphology of PVA fiber spinning, the diameter, such as PVA concentration, exploring the relationship between voltage
CONCLUSION: ① prepared by the observation of a few films The results showed that the concentration of spinning solution with the increase in fiber diameter increases, the distribution of fiber diameter increases; as the voltage increases, fiber diameter did not change significantly.
② experiment to make adequate preparations, phage with no spinning PVA polymer matrix to assess the compatibility.
[Key words]: phage titer PVA electrospinning
